Anti-CD5 Antibody [1H6/8] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD5

Applications

WB, IP, Flow Cytometry

Reactivity

Porcine

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to 1x10⁶ cells in 100µl

Clonă

1H6/8

Imunogen

Con A / PMA activated porcine peripheral blood cells.

Specificitate

This antibody recognises pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop , based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody. Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine ?/d T cells and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+). No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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