Anti-CD45 Antibody [UM16-6] (PE)

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Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

CD45

Applications

Flow Cytometry

Reactivity

Chicken

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

PE

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Protein G affinity chromatography of ascites.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Cross reactivity

This antibody does not cross react with Turkey

Reconstitution

Reconstitute with 1 ml of distilled water.

Clonă

UM16-6

Imunogen

COS cells transfected with CD45 gene.

Specificitate

This antibody recognises chicken CD45, also known as leucocyte common antigen. CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages. CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. De-phosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn.Chicken CD45 has an additional cysteine residue near the transmembrane region compared to human and shark CD45. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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