Anti-CD45 Antibody [2B11 + PD7/26] - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD45

Applications

Flow Cytometry, WB, IHC-P

Reactivity

Human, Canine

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Conjugate

Unconjugated

Host

Mouse

Clonality

Oligoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1, kappa + IgG1, kappa

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

180-220 kDa

Clonă

2B11 + PD7/26

Imunogen

Clone 2B11: Isolated neoplastic cells from T cell lymphoma. Clone PD7/26: Human peripheral blood lymphocytes maintained in T cell growth factor.

Specificitate

This antibody recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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