Anti-CD44 Antibody [25.32]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD44

Applications

IP, Flow Cytometry, IHC-Fr

Reactivity

Sheep, Bovine, Goat, Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 1 x 10⁶ cells in 100µl

Clonă

25.32

Imunogen

Sheep efferent lymphatic duct lymphocytes.

Specificitate

This antibody recognises the ovine CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by most leucocytes and a subpopulation of thymocytes. In immunohistochemical staining, this antibody labels several cell types including medullary thymocytes, kupffer cells, kidney tubular epithelial cells and gut smooth muscle cells. The expression of CD44 is upregulated upon cell activation. Mouse anti Sheep CD44 antibody, clone 25.32 is a valuable ragent for the isolation and characterization of ovine mesenchymal stem cells along with CD29 and CD166 which are also expressed on this cell type, in contrast to hematopoietic sell markers including CD45 which is negative on this mesenchymal stem sell population (Sanjurjo-Rodríguez et al. 2017).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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