Anti-CD40L Antibody [2A3]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

200µg

Țintă

CD40L

Applications

WB, ELISA 

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Clonă

2A3

Imunogen

Recombinant human soluble CD154 conjugated to Bovine Serum Albumin.

Specificitate

This antibody recognises the soluble form of CD154, otherwise known as CD40 Ligand/TRAP, a ~32 kDa glycoprotein and member of the TNF family, which is specifically expressed on activated, but not resting, CD4+ T cells, dendritic cells (DCs), B cells and macrophages. CD154 binds CD40, and acts as a key co-stimulatory molecule through the activation of antigen-presenting cells, and as a regulator of the generation and functional properties of Th1 helper cells. CD154 exists in both a membrane-bound and soluble form, the latter of which is generated through proteolytic processing by a matrix metalloproteinase (MMP), in activated cells.The binding of CD154 with its receptor CD40 is vital for many biological processes, including Ig class switching, germinal centre formation and the survival of CD40+ B cells, B cell proliferation in the absence of co-stimulus, the production of IgE in the presence of interleukin-4 (IL-4), and also in the production of pro-inflammatory cytokines, MMPs, prostaglandins and adhesion molecule upregulation, in non-immune cells. recognises the soluble form of the protein.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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