Anti-CD4 Antibody [CA13.1E4]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

2ml

Țintă

CD4

Applications

IHC-Fr, IP, Flow Cytometry

Reactivity

Canine

Formulation

Supplied in <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat, Use 25µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Purity

Tissue culture supernatant.

Clonă

CA13.1E4

Imunogen

Canine Thymocytes.

Specificitate

This antibody recognises a population of CD8 positive cells in the canine thymus. CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8 (Bismarck et al. 2012 ). Similar reports have been made for cynomolgus monkey , pig (Saalmuller et al. 1987 , Pescowitz et al. 1990) as well as rat, chicken and human (reviewed Zuckermann 1999 ).Uniquely amongst mammalian species clone CA13.1E4 also recognises CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species. Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone macrophages. Other macrophage populations, such as splenic red pulp macrophages and Langerhans cells do not stain with clone CA13.1E4.Canine CD4 positive T cells can be further characterized according to their simultaneous expression of CD45RA as recognized by Rat anti Canine anti CD45RA clone CA4.1D3 in a manner analogous to that seen with human T cells. Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for the study of various lymphoproliferative diseases. T lymphocytes in large granular lymphocyte (LGL) lymphocytosis are negative for CD4 (McDonough and Moore 2000 ). Dogs with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils. CD4 expression varies considerably between various canine leukemias.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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