Anti-CD3 epsilon Antibody [UCHT1] (APC)

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

CD3 epsilon

Applications

Flow Cytometry

Reactivity

Human, Chimpanzee

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Conjugate

APC

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Reconstitution

Reconstitute with 1 ml of distilled water.

Clonă

UCHT1

Imunogen

Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.

Specificitate

This antibody recognises the human T-cell surface glycoprotein CD3 epsilon chain, also known as T-cell surface antigen T3/Leu-4 epsilon chain or CD3e. CD3e is a 207 amino acid, ~21kDa single pass type 1 transmembrane protein containing a single Ig-like and a single ITAM domain. Mouse anti Human CD3 antibody, clone UCHT1 was originally described as only binding to CD3e when complexed with either the CD3d or CD3? subunits, as indicated by co-transfection immunofluorescence on COS cells (Salmerón et al. 1991). Mouse anti Human CD3 antibody, clone UCHT1 binds to a region in the ectodomain of human CD3e and binds to a discontinuous epitope near an acidic region of CD3e opposite the dimer interface; as shown by crystallography of the CD3e/d dimer complexed with a single chain UCHT1 antibody fragment. CD3 is expressed by all T lymphocytes and is seen in all lymphoid organs including lymph nodes and spleen. It is involved in thymocyte differentiation. Deficiency of the CD3e chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency phenotype. Mouse anti Human CD3 antibody, clone UCHT1 has been used successfully for the activation of human peripheral blood lymphocytes by cross linking and subsequently for CD3e surface espression by flow cytometry.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului