Anti-CD3 epsilon Antibody [145-2C11]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25µg · 100µg · 250µg

Țintă

CD3 epsilon

Applications

WB, IP, Flow Cytometry, IHC-Fr, IF

Reactivity

Mouse

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Hamster

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG

Recommended dilutions

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Clonă

145-2C11

Imunogen

H-2Kb specific mouse cytotoxic T lymphocyte clone BM10-37.

Specificitate

This antibody recognises CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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