Anti-CD3 Antibody [CA17.2A12] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD3

Applications

Flow Cytometry

Reactivity

Canine

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of ascites.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood. Note: MCA1774F should not be used with MCA1781PE (mouse anti canine B-cells), in dual colour FC, due to non-specific interactions between the two reagents.

Clonă

CA17.2A12

Imunogen

Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90.

Specificitate

This antibody recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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