Anti-CD28 Antibody [CC219] (PE)

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Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

CD28

Applications

Flow Cytometry

Reactivity

Bovine

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Conjugate

PE

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Reconstitution

Reconstitute with 1 ml of distilled water.

Clonă

CC219

Imunogen

Plasmid DNA encoding the bovine CD28 gene.

Specificitate

This antibody recognises the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells. Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional assays it is recommended to use the low endotoxin and preservative free format of this antibody, MCA5779EL. Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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