Anti-CD27 Antibody [B30C7] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD27

Applications

Flow Cytometry

Reactivity

Porcine

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Clonă

B30C7

Imunogen

Porcine Peripheral Blood Monocytes.

Specificitate

This antibody recognises the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however, using the B30C7 clone, expression of CD27 on porcine B-cells appears undetectable. Porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells. Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and clone B30C7 may be used to differentiate between subsets of pig NK cells.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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