Anti-CD21 Antibody [CC51]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD21

Applications

Flow Cytometry

Reactivity

Bovine, Pig, African Buffalo

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Clonă

CC51

Imunogen

Bovine (Friesian cattle) mesenteric lymph node cells.

Specificitate

This antibody recognises the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells. CD21 may be expressed on B-cells as either a long or a short form. Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkora et. al. 2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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