Anti-CD19 Antibody [LE-CD19]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 200µg

Țintă

CD19

Applications

WB, IHC-P, Flow Cytometry, ELISA, IP

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

FC 1: 1:100 - 1:200, IHC-P 2: 1:100 - 1:200, WB: Reducing conditions, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Clonă

LE-CD19

Imunogen

CD19 peptide CGPDPAWGGGGRMGTWSTR (C-terminus) coupled to Keyhole Limpet Haemocyanin.

Specificitate

This antibody recognises an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region.Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and proliferation.B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms. B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common components of both active and chronic MS lesions and well as the CSF Mouse anti Human CD19 antibody, clone LE-CD19 has been successfully employed for the immunohistochemical demonstration of CD19 in formalin fixed, paraffin embedded tissues (Streeck, H. et al. 2011) and for the detection of CD19 in cell lysates by Western blotting.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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