Anti-CD18 Antibody [CA1.4E9]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD18

Applications

IP, Flow Cytometry, IHC-Fr, IF

Reactivity

Canine, Horse, Pig, Human, Bovine, Cat, Mustelid, Mink, Hooded Seal

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of ascites.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Clonă

CA1.4E9

Specificitate

This antibody recognises the canine CD18 cell surface antigen, also known as the b2 integrin.The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of recognises an epitope common to a number of mammalian species.Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp. High CD18 expression is a common feature of lymphoma and hyperplasia in dogs

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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