Anti-Caldesmon Antibody - BSA and Azide free

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Caldesmon

Applications

WB, Flow Cytometry, IF, IHC

Reactivity

Human, Rat

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A/G chromatography.

Isotype

IgG

Recommended dilutions

WB: 1-2 µg/ml, Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

120-150 kDa

Imunogen

Recombinant fragment of human CALD1 protein.

Specificitate

This antibody recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody This antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului