Anti-C3a Antibody [048-18.6.3.X (10-21)]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µl

Țintă

C3a

Applications

ELISA, WB

Reactivity

Human

Formulation

Supplied in Borate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

Lot Specific

Purification

Protein A affinity chromatography of ascites.

Isotype

IgG1

Recommended dilutions

ELISA: 1:1500 - 1:6,000

Clonă

048-18.6.3.X (10-21)

Imunogen

Native purified human C3a.

Specificitate

This antibody recognises the ~9 kDa complement component 3a (C3a) which is present in the blood serum. C3a is generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3a and C3b, which is further degraded into iC3b and C3dg/C3d. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer. Mouse anti Human C3a antibody, clone 10-21 shows minimal reactivity with other complement proteins in radioimmunoassays and haemagglutination. Mouse anti Human C3a antibody, clone 10-21 has been reported to work in western blotting applications.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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