Anti-BrdU Antibody [Bu20a]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 100µg · 200µg

Țintă

BrdU

Applications

IHC-P, Flow Cytometry, IHC-Fr, IF, ICC

Reactivity

Species independent

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

FC 1: 1:25 - 1:100, ICC 2: 1:25 - 1:100, Use 10 µl of the suggested working dilution to label 1x10⁶ cells in 100 µl

Clonă

Bu20a

Imunogen

Bromodeoxyuridine conjugated to Bovine Serum Albumin.

Specificitate

This antibody recognises bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. recognises BrdU and other thymidine analogs; 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989).Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants or in drinking water. BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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