Anti-BRCA1 Antibody [17F8]

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Descriere

Specificații

Brand

Antibodies

Mărime

50µg

Țintă

BRCA1

Applications

ELISA, WB, IHC-P, IP, IF

Reactivity

Human, Mouse

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of ascites.

Isotype

IgG1

Recommended dilutions

IF: 1 µg/ml, WB: 1.0 - 10.0 µg/ml

Protein Length

554

Clonă

17F8

Secvență

RSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDP

Imunogen

E. coli derived recombinant BRCA1 protein fragment corresponding to amino acids 762-1315.

Aminoacizi

aa 762 - 1315

Specificitate

This antibody recognises both human and recognises full length 220 kDa BRCA1, but not the delta exon 11 splice variant. Mouse anti BRCA1, clone17F8 is well documented for use in immunohistochemistry and immunofluorescence staining, showning that this antibody labels both cytoplasmic BRCA1 and the centromeric region (overlapping with the nucleus), and exhibits cytoplasmic staining of BRCA1 in breast cancer cell lines and nuclear staining in several non-breast cancer cell lines (Lee, et al. 1998).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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