Anti-Amyloid Precursor Protein Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Amyloid Precursor Protein

Applications

WB, IHC-P

Reactivity

Human, Rat, (Mouse)

Formulation

Supplied in phosphate-buffered saline with 0.02% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

1 mg/ml

Isotype

IgG

Recommended dilutions

WB: 1:500-1:1,000

Protein Length

15

Secvență

EVDAAVTPEERHLSK

Imunogen

Synthetic peptide corresponding to amino acids 737-751 of human APP.

Aminoacizi

aa 737 - 751

Specificitate

This antibody recognises both intact Amyloid Precursor Protein (APP), and also the C99 fragment generated by beta-secretase. The sequence recognized by this antibody corresponds to amino acids 85-99 of the C99 fragment. The C99 fragment itself is a substrate for gamma-secretase to generate the 4 kDa beta amyloid peptide, found in the brains of Alzheimer's disease patients. APP also inhibits Notch signaling through it’s interaction with NUMB. Rabbit anti Amyloid Precursor Protein antibody has been used successfully for the detection of the ~14 kDa ß C-terminal fragment of APP, produced as a result of cleavage by BACE1 using western blotting -in rat retinal lysates.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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