Anti-alpha Tubulin Antibody [YL1/2]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

alpha Tubulin

Applications

WB, IF, IP, IHC-Fr, ELISA, RIA

Reactivity

S. cerevisiae, Ashbya, Birds, Echinoderm, Human, Mouse, Dog, Rat, Pig, Drosophila, Plants, Amphibia, Saccharomyces, Pleurobrachia, Caenorhabditis, Dictyostelium discoideum, Xenopus, Pig-tailed macaque, Clytia sp., Arabidopsis, Strongylocentrotus purpuratus, Dendraster excentricus, Trypanosoma brucei, Potorous tridactylis, Bovine, Hemicentrotus pulcherrimus, Potato, Bombyx mori, Rhodnius prolixus, Beroe abyssicola, Candida sp.

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Rat

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

ELISA: 1:100 - 1:1,000, WB: 1:200 - 1:2,000

Molecular weight

Approximately 55 kDa in cell lysate from a wide range of species. Rat anti tubulin alpha antibody, clone YL1/2 is suitable for use as a Western Blotting loading control.

Cross reactivity

This antibody does not cross react with Nephrotoma suturalis

Clonă

YL1/2

Imunogen

Yeast tubulin.

Specificitate

This antibody recognises the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987). Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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