Anti-alpha Synuclein (phospho Ser129) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µl

Țintă

alpha Synuclein

Applications

WB

Reactivity

Rat, Mouse, Dog, Primate, Human, Bovine

Formulation

Supplied in 10mM HEPES, pH 7.5, with 150mM NaCl, 0.01% BSA, 50% Glycerol, and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

WB: 1:1,000

Molecular weight

Approximately 15 kDa in rat cortex lysate.

Imunogen

Synthetic phosphopeptide corresponding to an amino acid sequence within alpha Synuclein which include phosphorylated Ser129.

Specificitate

This antibody recognises alpha synuclein when phosphorylated at serine 129. Alpha synuclein is a 140 amino acid, ~15 kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal.Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, serine 129 in particular can be phosphorylated by G-protein-coupled receptor kinases and this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of serine129 seen in Lewy bodies.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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