Amino Anchor Immunoprecipitation to Mass Spectrometry Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

8 Reactions

Applications

IP, Mass Spec

Product form

Liquid and Resin

Sample type

Serum, plasma, or other antibody source, and cell lysate, tissue lysate or other protein source

Principiul testului

The Amino Anchor Immunoprecipitation to Mass Spectrometry Kit uses the bifunctional chemistry of the Amino Anchor to prepare pure, high-quality antigens for unbiased antigen profiling via mass spectrometry (MS). This approach is particularly useful for the identification of autoantigens and cancer antigens in a sample-specific manner using only a small volume of plasma or serum (or other auto-antibody source). First, antigens are labeled with the Amino Anchor via its amine-reactive group. Antibodies (including auto-antibodies) from a single sample are bound to Protein A resin, then used to capture target antigens. After washing away unbound proteins, antigens are eluted and recaptured on TCO resin via the resin-reactive group of the Amino Anchor, resulting in antibody removal. Pure target antigens can then be eluted for use or digested with trypsin for MS analysis without the contaminating signal from overabundant antibodies. This simplified procedure generates MS-ready peptides in as little as 6 hours, is radiolabel-free, and is compatible with a range of antibody and target antigen sources.

Storage

Store Protein A resin and Amino Anchor capture resin at 4°C. Store Amino Anchor, Quencher, and MT-Trypsin at -20°C. Other components may be stored at room temperature.