# TAMRA phosphoramidite, 5-isomer

**Cod produs:** A270325 · **Brand:** Antibodies · **Categorie:** Assistive Reagents

TAMRA phosphoramidite, 5-isomer este un produs din categoria Assistive Reagents, marca Antibodies, cod A270325. Se livrează în mărimile 250mg și 1g, la prețuri între 2.961,48 și 6.455,35 lei cu TVA. Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.

## Specificații

| Caracteristică | Valoare |
| --- | --- |
| Product form | Purple solid. |
| Molecular weight | 727.83 Da |
| Purity | 95% (by 1H NMR, HPLC-MS, and coupling testing). |
| Extinction coefficient | 84000 M-1cm-1 |
| Emission maxima | 567 nm |
| Solubility | Good in acetonitrile. |
| Absorption maxima | 541 nm |
| CF260 | 0.32 |
| CF280 | 0.19 |
| Storage | Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate. |
| Formulă moleculară | C40H50N5O6P |

## Mărimi și prețuri

| Mărime | Cod și preț (RON, TVA inclus) |
| --- | --- |
| 250mg | A270325-250 · 2.961,48 lei |
| 1g | A270325-1 · 6.455,35 lei |

## Descriere

This phosphoramidite is used for synthesis of oligonucleotides 5'-labeled with TAMRA. TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5'-terminal TAMRA and FAM in the middle of the sequence or at the 3'-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively). TAMRA 5'-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently. The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection. Coupling takes only 7.5 minutes. Deprotection should be performed with tret-buthylamine : methanol : water at a ratio of 1 : 1 : 3 (v/v/v) ("TAMRA cocktail") for 6 hours at 60 °C, then cooled down to room temperature. Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.

## Documentație

- [Fișă tehnică (PDF)](https://www.antibodies.com/datasheets/270/A270325.pdf)

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Sursă: https://reactivi.ro/produs/tamra-phosphoramidite-5-isomer/ · actualizat 9 septembrie 2026