# EU (5-ethynyl uridine)

**Cod produs:** A270203 · **Brand:** Antibodies · **Categorie:** Assistive Reagents

EU (5-ethynyl uridine) este un produs din categoria Assistive Reagents, marca Antibodies, cod A270203. Se livrează în mărimile 25mg și 500mg, la prețuri între 1.331,00 și 7.253,95 lei cu TVA. Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.

## Specificații

| Caracteristică | Valoare |
| --- | --- |
| Product form | Off white solid. |
| Molecular weight | 268.22 Da |
| Purity | 95% (by 1H NMR and HPLC-MS). |
| Solubility | Good in water, DMSO, and DMF. |
| Storage | Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. |
| Cas number | 69075-42-9 |
| Formulă moleculară | C11H12N2O6 |

## Mărimi și prețuri

| Mărime | Cod și preț (RON, TVA inclus) |
| --- | --- |
| 25mg | A270203-25 · 1.331,00 lei |
| 500mg | A270203-500 · 7.253,95 lei |

## Descriere

5-ethynyl uridine (EU) is a commonly used molecular biology reagent for RNA synthesis studies. EU is readily taken up by living cells and phosphorylated through the pyrimidine salvage pathway. Generated 5-ethynyl uridine-5’-triphosphate is incorporated by RNA polymerases I, II, and III into de novo RNA instead of uridine, but not into DNA. EU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via Click Chemistry following fluorescent visualization. Alkyne group attached at the 5-position of uridine in modified RNA reacts with dye or biotin azides via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). Labeled RNA can be detected with different methods, e.g. fluorescent microscopy or flow cytometry, which allows estimating transcriptional levels in the cells. 5-ethynyl uridine has an advantage over its analog, 5-bromouridine, because azide-containing dyes are very small in size and exhibit better membrane permeability compared to antibodies used for the detection of 5-bromouridine. Thus, the Click Chemistry approach allows whole-mount staining of large samples like organs or tissue fragments.

## Documentație

- [Fișă tehnică (PDF)](https://www.antibodies.com/datasheets/270/A270203.pdf)

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Sursă: https://reactivi.ro/produs/eu-5-ethynyl-uridine/ · actualizat 9 septembrie 2026