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Rat DOPA Decarboxylase / DDC ELISA Kit (A303750-96)
Cod: A303750-96
Rat DOPA Decarboxylase / DDC ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat DOPA Decarboxylase / DDC in serum, plasma, tissue homogenates, and other biological fluids.
Numar teste:
96T
3.960,32 Lei
Timp de livrare: 2-3 saptamani
Preturile includ TVA 19%
Rat DOPA Decarboxylase / DDC ELISA Kit (A303750) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat DOPA Decarboxylase / DDC in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for DOPA Decarboxylase / DDC has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the DOPA Decarboxylase / DDC present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-DOPA Decarboxylase / DDC Antibody, which binds the captured DOPA Decarboxylase / DDC present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of DOPA Decarboxylase / DDC captured in each well. The concentration of DOPA Decarboxylase / DDC can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
- Serum, plasma, tissue homogenates, and other biological fluids.
- ANTIBODIES
- Sandwich (quantitative)
- 31.25-2000 pg/ml
- 18.75 pg/ml
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