Poly (ADP-Ribose) Polymer ELISA Kit (A303880) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Poly (ADP-Ribose) Polymer in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Poly (ADP-Ribose) Polymer antigen. During the incubation, Poly (ADP-Ribose) Polymer present in the samples or standards competes with the fixed amount of immobilized Poly (ADP-Ribose) Polymer for binding sites on the Biotinylated Anti-Poly (ADP-Ribose) Polymer Antibody. The more Poly (ADP-Ribose) Polymer present in a sample or standard, the less Biotinylated Anti-Poly (ADP-Ribose) Polymer Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Poly (ADP-Ribose) Polymer Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Poly (ADP-Ribose) Polymer present in each sample or standard. The concentration of Poly (ADP-Ribose) Polymer can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.