This p38 MAPK (phospho Thr180) Cell Based ELISA Kit allows for the detection of p38 MAPK (phospho Thr180) and the effects that certain stimulation conditions have on p38 MAPK (phospho Thr180) expression in different cell lines. Qualitative determination of p38 MAPK (phospho Thr180) concentration is achieved by an indirect ELISA format. In essence, p38 MAPK (phospho Thr180) is captured by Anti-p38 MAPK (phospho Thr180) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-p38 MAPK Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.