Mouse D Amino Acid Oxidase ELISA Kit (A77969) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse D Amino Acid Oxidase in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for D Amino Acid Oxidase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the D Amino Acid Oxidase present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-D Amino Acid Oxidase Antibody, which binds the captured D Amino Acid Oxidase present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of D Amino Acid Oxidase captured in each well. The concentration of D Amino Acid Oxidase can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.