Mouse BPI ELISA Kit (A319508) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse BPI in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for BPI has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the BPI present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-BPI Antibody, which binds the captured BPI present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of BPI captured in each well. The concentration of BPI can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.