Mouse BMP1 / PCP ELISA Kit (A313110) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse BMP1 / PCP in serum, plasma or other biological fluids. An antibody specific for BMP1 / PCP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the BMP1 / PCP present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-BMP1 / PCP Antibody, which also binds the BMP1 / PCP present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-BMP1 / PCP Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-BMP1 / PCP Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of BMP1 / PCP captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of BMP1 / PCP in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.