Mouse Angiotensin Converting Enzyme 1 ELISA Kit (A312598) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Angiotensin Converting Enzyme 1 in serum, plasma or other biological fluids. An antibody specific for Angiotensin Converting Enzyme 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Angiotensin Converting Enzyme 1 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Angiotensin Converting Enzyme 1 Antibody, which also binds the Angiotensin Converting Enzyme 1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Angiotensin Converting Enzyme 1 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Angiotensin Converting Enzyme 1 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Angiotensin Converting Enzyme 1 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Angiotensin Converting Enzyme 1 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.