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Human xCT ELISA Kit (A311593-96)
Cod: A311593-96
Human xCT ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human xCT in serum, plasma, and other biological fluids.
Numar teste:
96T
3.434,34 Lei
Timp de livrare: 2-3 saptamani
Preturile includ TVA 19%
Human xCT ELISA Kit (A311593) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human xCT in An antibody specific for xCT has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the xCT present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-xCT Antibody, which also binds the xCT present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-xCT Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-xCT Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of xCT captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of xCT in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
- Serum, plasma or other biological fluids.
- ANTIBODIES
- Sandwich (quantitative)
- 75-1200 ng/L
- 4.36 ng/L
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