Human TPSD1 ELISA Kit (A313134) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human TPSD1 in An antibody specific for TPSD1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TPSD1 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-TPSD1 Antibody, which also binds the TPSD1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-TPSD1 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-TPSD1 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of TPSD1 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of TPSD1 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.