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Human TIP49A ELISA Kit (A313267-96)
Cod: A313267-96

Human TIP49A ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TIP49A in serum, plasma, and other biological fluids.

Numar teste: 96T
3.434,34 Lei
Timp de livrare: 2-3 saptamani
Preturile includ TVA 19%
Cere oferta
Human TIP49A ELISA Kit (A313267) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human TIP49A in An antibody specific for TIP49A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TIP49A present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-TIP49A Antibody, which also binds the TIP49A present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-TIP49A Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-TIP49A Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of TIP49A captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of TIP49A in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
  • Serum, plasma or other biological fluids.
  • ANTIBODIES
  • Sandwich (quantitative)
  • 100-1600 ng/L
  • 7.15 ng/L
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