Human Thyroid Hormone Receptor alpha ELISA Kit (A310131) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Thyroid Hormone Receptor alpha in An antibody specific for Thyroid Hormone Receptor alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Thyroid Hormone Receptor alpha present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Thyroid Hormone Receptor alpha Antibody, which also binds the Thyroid Hormone Receptor alpha present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Thyroid Hormone Receptor alpha Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Thyroid Hormone Receptor alpha Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Thyroid Hormone Receptor alpha captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Thyroid Hormone Receptor alpha in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.