Human SULT1A1 / STP ELISA Kit (A312705) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human SULT1A1 / STP in An antibody specific for SULT1A1 / STP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SULT1A1 / STP present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-SULT1A1 / STP Antibody, which also binds the SULT1A1 / STP present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-SULT1A1 / STP Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-SULT1A1 / STP Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of SULT1A1 / STP captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of SULT1A1 / STP in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.