Human Junctional Adhesion Molecule 2 / JAM-B ELISA Kit (A313429) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Junctional Adhesion Molecule 2 / JAM-B in serum, plasma or other biological fluids. An antibody specific for Junctional Adhesion Molecule 2 / JAM-B has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Junctional Adhesion Molecule 2 / JAM-B present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Junctional Adhesion Molecule 2 / JAM-B Antibody, which also binds the Junctional Adhesion Molecule 2 / JAM-B present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Junctional Adhesion Molecule 2 / JAM-B Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Junctional Adhesion Molecule 2 / JAM-B Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Junctional Adhesion Molecule 2 / JAM-B captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Junctional Adhesion Molecule 2 / JAM-B in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.