Human Insulin Degrading Enzyme / IDE ELISA Kit (A312256) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Insulin Degrading Enzyme / IDE in serum, plasma or other biological fluids. An antibody specific for Insulin Degrading Enzyme / IDE has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Insulin Degrading Enzyme / IDE present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Insulin Degrading Enzyme / IDE Antibody, which also binds the Insulin Degrading Enzyme / IDE present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Insulin Degrading Enzyme / IDE Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Insulin Degrading Enzyme / IDE Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Insulin Degrading Enzyme / IDE captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Insulin Degrading Enzyme / IDE in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.