This ELISA kit is indirect enzyme-linked immune-sorbent assay technology. The recombinant HPV16 L1 antigens were pre-coated onto 96-well plates. The test samples were added to the wells and incubated subsequently, the HPV16 L1 antibodies in the test samples combined to the pre-coated antigens specifically, then followed by washing with wash buffer to remove unspecific components. Next, the HRP conjugated antibodies were added and incubated to bind to human antibodies in plate, the unbound conjugates were washed away with wash buffer. TMB substrate were used to visualize HRP enzymatic reaction. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.