Human HAO1 / GOX ELISA Kit (A312674) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human HAO1 / GOX in serum, plasma or other biological fluids. An antibody specific for HAO1 / GOX has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HAO1 / GOX present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-HAO1 / GOX Antibody, which also binds the HAO1 / GOX present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-HAO1 / GOX Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-HAO1 / GOX Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of HAO1 / GOX captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of HAO1 / GOX in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.