Human FAM214A ELISA Kit (A74786) employs the competitive enzyme immunoassay technique for the quantitative measurement of human FAM214A in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with FAM214A antigen. During the incubation, FAM214A present in the samples or standards competes with the fixed amount of immobilized FAM214A for binding sites on the Biotinylated Anti-FAM214A Antibody. The more FAM214A present in a sample or standard, the less Biotinylated Anti-FAM214A Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-FAM214A Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of FAM214A present in each sample or standard. The concentration of FAM214A can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.