Human Deoxypyridinoline ELISA Kit (A78002) employs the competitive enzyme immunoassay technique for the quantitative measurement of human Deoxypyridinoline in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Deoxypyridinoline antigen. During the incubation, Deoxypyridinoline present in the samples or standards competes with the fixed amount of immobilized Deoxypyridinoline for binding sites on the Biotinylated Anti-Deoxypyridinoline Antibody. The more Deoxypyridinoline present in a sample or standard, the less Biotinylated Anti-Deoxypyridinoline Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Deoxypyridinoline Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Deoxypyridinoline present in each sample or standard. The concentration of Deoxypyridinoline can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.