Human B3GALT2 ELISA Kit (A74673) employs the competitive enzyme immunoassay technique for the quantitative measurement of human B3GALT2 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with B3GALT2 antigen. During the incubation, B3GALT2 present in the samples or standards competes with the fixed amount of immobilized B3GALT2 for binding sites on the Biotinylated Anti-B3GALT2 Antibody. The more B3GALT2 present in a sample or standard, the less Biotinylated Anti-B3GALT2 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-B3GALT2 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of B3GALT2 present in each sample or standard. The concentration of B3GALT2 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.