This assay applies an enzyme-linked immunosorbent assay (ELISA) technique. A purified mouse IgG has been pre-coated onto the wells of microplate. Standard (rabbit anti-mouse IgG) and specimens are added into the appropriate wells simultaneously with a HRP-labelled mouse IgG, and incubated. The Human Anti-Mouse Antibody (HAMA), if present, will bind with the mouse IgG which coated on the wells and HRP-labelled mouse IgG as well, forming a "Sandwich". Following a wash to remove any unbound substance, a substrate solution is added. The developed colour is directly proportional to the anti-mouse antibody concentration in the specimen and Standard samples.

This ELISA Kit includes a Calibration Diluent for preparing a 2-fold dilution of Standard series. It allows the operator to produce a standard curve with Optical Density (O.D) versus Human Anti-Mouse Antibody concentration (ng/mL). The concentration of Human Anti-Mouse Antibody in the sample is then determined by comparing the O.D. of the sample to the standard curve.