This eNOS (phospho Ser1176) Cell Based ELISA Kit allows for the detection of eNOS (phospho Ser1176) and the effects that certain stimulation conditions have on eNOS (phospho Ser1176) expression in different cell lines. Qualitative determination of eNOS (phospho Ser1176) concentration is achieved by an indirect ELISA format. In essence, eNOS (phospho Ser1176) is captured by Anti-eNOS (phospho Ser1176) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-eNOS Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.